Ethidium Bromide Solid and Stock Solutions
Stock solutions typically contain higher concentrations (approximately 10 mg/ml) of ethidium bromide. Outdated or unwanted ethidium bromide powder, tablets, or stock solutions should be referred to EHS for chemical waste disposal.
Electrophoresis gels contaminated with ethidium bromide should be referred to EHS for
chemical waste disposal.
Leak proof containers must be used to store ethidium bromide gels for disposal. Ziploc makes an inexpensive and leak proof container (Figure 1) acceptable for storage and transportation of ethidium bromide. Plastic pails with a tight fitting lid are also a good option. Bag lined cardboard boxes or Styrofoam containers arenot acceptable.
Buffers containing less than 0.5 ug/ml of ethidium bromide, and not contaminated with other hazardous materials can be drain disposed.
Ethidium bromide contaminated sharps such as pipette tips, should be placed in a separate sharps container and disposed of through EHS.